EAE animal model


Protocol of inducing


6-8-week-old healthy female C57BL/6 mice were immunized by MOG35-55/CFA on day0. 

验证数据-EAE小鼠模型-图1.png

Validation data

验证数据-EAE小鼠模型-图2.png

Fig 1. Phenotype Score of EAE disease from 1 to 4

Score index

0, healthy;

1, tail paralysis;

2, impaired righting reflex 

3, one of hind limbs paralysis; 

4, both of hind limbs paralysis;

5, weight loss more than 20%; 

6, death.

验证数据-EAE小鼠模型-图3.png

Fig 2. EAE score from day0 to day30

验证数据-EAE小鼠模型-图4.png

Fig 3. The cumulative score for each EAE mice from day0 to day30

验证数据-EAE小鼠模型-图5.png

Fig 4. The day of onset of disease for each mice immunized

验证数据-EAE小鼠模型-图6.png

Fig 5. The day of peak of disease for each mice immunized


Conclusion

  1. The day of onset of disease focus on day10 to day12 after immunization on day0.

  2. The day of peak of disease is around on day17 and the cycle of disease is about 30 days.


Popular articles

Workshop:Progress and Advances in Preclinical immuno-Oncology Research

SMOC’s Annual Progress and Advances in Preclinical immuno-Oncology Research: The workshop is designed as a forum for ideas and opinions exchange on how to decrease the rate of clinical failures in oncology and immuno-oncology.

查看
Shanghai Model Organisms Jinshan R&D Base officially put into operation

After the base is put into operation, SMOC’s capability to provide genetically modified rat/mouse models and technical services including gene function research and drug development will be greatly enhanced.

查看